C-TAK1 interacts with microphthalmia-associated transcription factor, Mitf, but not the related family member Tfe3

Characterizing the Coding Region Determinant-Binding Protein (CRD-BP)-Microphthalmia-associated Transcription Factor (MITF) mRNA interaction

Coding region determinant-binding protein (CRD-BP) binds to the 3'-UTR of microphthalmia-associated transcription factor (MITF) mRNA to prevent its targeted degradation by miR-340. Here, we aim to further understand the molecular interaction between CRD-BP and MITF RNA. Using point mutation in the GXXG motif of each KH domains, we showed that all four KH domains of CRD-BP are important for thei...

In vivo role of alternative splicing and serine phosphorylation of the microphthalmia-associated transcription factor MITF

*Mammalian Development Section, NINDS, NIH, Bethesda, MD 20892-3706, USA §Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH, Bethesda, MD 20892, USA **Genetics Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA §§NINDS Flow Cytometry Core Facility, NIH, Bethesda, MD 20892, USA Transgenic Core Facility, National Institute of Mental Health, National Institutes...

The Microphthalmia-associated Transcription Factor (mitf) Requires Swi/snf Enzymes to Activate Melanocyte Specific Genes

Inhibition of NAT10 Suppresses Melanogenesis and Melanoma Growth by Attenuating Microphthalmia-Associated Transcription Factor (MITF) Expression

N-acetyltransferase 10 (NAT10) has been considered a target for the treatment of human diseases such as cancer and laminopathies; however, its functional role in the biology of melanocytes is questionable. Using a small molecule or small interfering RNA targeting NAT10, we examined the effect of NAT10 inhibition on melanogenesis and melanoma growth in human and mouse melanoma cells. Genetic sil...

SOX9, through interaction with microphthalmia-associated transcription factor (MITF) and OTX2, regulates BEST1 expression in the retinal pigment epithelium.

BEST1 is highly and preferentially expressed in the retinal pigment epithelium (RPE) and causes Best macular dystrophy when mutated. We previously demonstrated that the human BEST1 upstream region -154 to +38 bp is sufficient to direct expression in the RPE of transgenic mice, and microphthalmia-associated transcription factor (MITF) and OTX2 regulate this BEST1 promoter. However, a number of q...